Purification and characterization of .BETA.-N-acetylhexosaminidase from Penicillium oxalicum.
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چکیده
منابع مشابه
Purification and Characterization of β-N-Acetylhexosaminidase from Rice Seeds
N-Acetyl-β-D-hexosaminidase (β-HexNAc’ase) (EC 3.2.1.52) was purified from rice seeds (Oryza sativa L. var. Dongjin) using ammonium sulfate (80%) precipitation, Sephadex G150, CM-Sephadex, and DEAE-Sephadex chromatography, sequentially. The activities were separated into 7 fractions (F1F7) by CM-Sephadex chromatography. Among them, F6 was further purified to homogeneity with a 13.0% yield and 1...
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beta-N-Acetylhexosaminidase I2 was purified from human liver by a combination of concanavalin A chromatography, DEAE-cellulose chromatography, gel filtration and affinity chromatography on 2-acetamido-N-(6-aminohexanoyl)-2-deoxy-beta-D-glucopyranosylamine coupled to CNBr-activated Sepharose 4B. Its specific activity was 130 mumol/min per mg of protein compared with values of 150 and 320 mumol/m...
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Three isoforms of β-N-acetylhexosaminidase (β-NAHA), named β-NAHAs I, II and III, were isolated from six-day-old etiolated mungbean (Vigna radiata) seedlings. β-NAHA I was purified to apparent homogeneity by a procedure involving Con A-Sepharose chromatography, chromatofocusing, and gel filtration. β-NAHAs II and III were highly purified. β-NAHAs I, II and III had molecular masses of 135,127 an...
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In this study, β-1, 4-endoglucanase from Penicillium simplicissimum H-11 was purified to homogeneity using ammonium sulfate followed by Sephadex G-100 chromatography. The purity of the enzyme was confirmed by HPLC and 12% SDS-PAGE, indicating a single peak with a molecular mass of 33.2 kDa. This protein had mostly α-helix structures, as confirmed by FTIR spectrometry. The optimum pH and tempera...
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Paucimannosidic glycans are often predominant in N-glycans produced by insect cells. However, a beta-N-acetylhexosaminidase responsible for the generation of paucimannosidic glycans in lepidopteran insect cells has not been identified. We report the purification of a beta-N-acetylhexosaminidase from the culture medium of Spodoptera frugiperda Sf9 cells (Sfhex). The purified Sfhex protein showed...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1985
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.49.611